منابع مشابه
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Corynebacterium glutamicum is an important organism for the industrial production of amino acids. Metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. To facilitate the mapping of gene expression levels to metabolic outputs, we applied CRISPR interference (CRISPRi) technology using dea...
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Bacteria of the order Actinomycetales are one of the most important sources of pharmacologically active and industrially relevant secondary metabolites. Unfortunately, many of them are still recalcitrant to genetic manipulation, which is a bottleneck for systematic metabolic engineering. To facilitate the genetic manipulation of actinomycetes, we developed a highly efficient CRISPR-Cas9 system ...
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As the focus of microbial metabolic engineering shifts to include bulk commodities, two aspects of this research have become increasingly important. One is the requirement for high production titers in order to make the process economically viable. The second is to engineer microbes that are tolerant to these target products, many of which are toxic to cell growth. This review focuses on recent...
متن کاملPune University Metabolic Pathway Engineering ( PuMPE ) Resource
PuMPE is a comprehensive resource that provides integrated information on metabolome of bacterial systems. The genome data is annotated to infer metabolic pathways using in-house tools and web-based sources. PuMPE introduces a novel aspect of metabolic categorization. It is the first resource to provide metabolome-based tree computed by comparing metabolome between bacteria. PuMPE has metabolic...
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ژورنال
عنوان ژورنال: Metabolic Engineering
سال: 2021
ISSN: 1096-7176
DOI: 10.1016/j.ymben.2020.10.004